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Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery.

ABSTRACT: We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.

SUBMITTER: McMahon KM

PROVIDER: S-EPMC4077779 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery.

McMahon Kaylin M KM Mutharasan R Kannan RK Tripathy Sushant S Veliceasa Dorina D Bobeica Mariana M Shumaker Dale K DK Luthi Andrea J AJ Helfand Brian T BT Ardehali Hossein H Mirkin Chad A CA Volpert Olga O Thaxton C Shad CS

Nano letters 20110214 3

We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemica ...[more]

PMID: 21319839

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