Ontology highlight
ABSTRACT:
SUBMITTER: Makharashvili N
PROVIDER: S-EPMC4079050 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Makharashvili Nodar N Tubbs Anthony T AT Yang Soo-Hyun SH Wang Hailong H Barton Olivia O Zhou Yi Y Deshpande Rajashree A RA Lee Ji-Hoon JH Lobrich Markus M Sleckman Barry P BP Wu Xiaohua X Paull Tanya T TT
Molecular cell 20140515 6
The carboxy-terminal binding protein (CtBP)-interacting protein (CtIP) is known to function in 5' strand resection during homologous recombination, similar to the budding yeast Sae2 protein, but its role in this process is unclear. Here, we characterize recombinant human CtIP and find that it exhibits 5' flap endonuclease activity on branched DNA structures, independent of the MRN complex. Phosphorylation of CtIP at known damage-dependent sites and other sites is essential for its catalytic acti ...[more]