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Detecting DNA depurination with solid-state nanopores.

ABSTRACT: Among the different types of DNA damage that occur endogenously in the cell, depurination is especially prevalent. These lesions can initiate mutagenesis and have been implicated in a variety of diseases, including cancer. Here, we demonstrate a new approach for the detection of depurination at the single-molecule scale using solid-state nanopores. We induce depurination in short duplex DNA using acidic conditions and observe that the presence of apurinic sites results in significantly slower dynamics during electrokinetic translocation. This procedure may be valuable as a diagnostic for in situ quantification of DNA depurination.

SUBMITTER: Marshall MM

PROVIDER: S-EPMC4079296 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Detecting DNA depurination with solid-state nanopores.

Marshall Michael M MM Ruzicka Jan A JA Taylor Ethan W EW Hall Adam R AR

PloS one 20140702 7

Among the different types of DNA damage that occur endogenously in the cell, depurination is especially prevalent. These lesions can initiate mutagenesis and have been implicated in a variety of diseases, including cancer. Here, we demonstrate a new approach for the detection of depurination at the single-molecule scale using solid-state nanopores. We induce depurination in short duplex DNA using acidic conditions and observe that the presence of apurinic sites results in significantly slower dy ...[more]

PMID: 24988437

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