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Nonenzymatic polymerization of ubiquitin: single-step synthesis and isolation of discrete ubiquitin oligomers.

ABSTRACT: Linked: a method based on thiol-ene chemistry enables the synthesis and purification of ubiquitin oligomers with ?4 units. This approach, which employs free-radical polymerization, can be applied towards the synthesis of homogeneous Lys6-linked ubiquitin oligomers currently inaccessible by enzymatic methods. By using these chains, one can study their roles in the ubiquitin proteasome system and the DNA damage response pathway.

SUBMITTER: Trang VH

PROVIDER: S-EPMC4083817 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Nonenzymatic polymerization of ubiquitin: single-step synthesis and isolation of discrete ubiquitin oligomers.

Trang Vivian H VH Valkevich Ellen M EM Minami Shoko S Chen Yi-Chen YC Ge Ying Y Strieter Eric R ER

Angewandte Chemie (International ed. in English) 20121119 52

Linked: a method based on thiol-ene chemistry enables the synthesis and purification of ubiquitin oligomers with ≥4 units. This approach, which employs free-radical polymerization, can be applied towards the synthesis of homogeneous Lys6-linked ubiquitin oligomers currently inaccessible by enzymatic methods. By using these chains, one can study their roles in the ubiquitin proteasome system and the DNA damage response pathway. ...[more]

PMID: 23161800

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