Ontology highlight
ABSTRACT:
SUBMITTER: Der BS
PROVIDER: S-EPMC4084500 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Der Bryan S BS Jha Ramesh K RK Lewis Steven M SM Thompson Peter M PM Thompson Peter M PM Guntas Gurkan G Kuhlman Brian B
Proteins 20130420 7
We computationally designed a de novo protein-protein interaction between wild-type ubiquitin and a redesigned scaffold. Our strategy was to incorporate zinc at the designed interface to promote affinity and orientation specificity. A large set of monomeric scaffold surfaces were computationally engineered with three-residue zinc coordination sites, and the ubiquitin residue H68 was docked to the open coordination site to complete a tetrahedral zinc site. This single coordination bond was intend ...[more]