Ontology highlight
ABSTRACT:
SUBMITTER: Li X
PROVIDER: S-EPMC4087144 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Li Xingnan X Nadauld Lincoln L Ootani Akifumi A Corney David C DC Pai Reetesh K RK Gevaert Olivier O Cantrell Michael A MA Rack Paul G PG Neal James T JT Chan Carol W-M CW Yeung Trevor T Gong Xue X Yuan Jenny J Wilhelmy Julie J Robine Sylvie S Attardi Laura D LD Plevritis Sylvia K SK Hung Kenneth E KE Chen Chang-Zheng CZ Ji Hanlee P HP Kuo Calvin J CJ
Nature medicine 20140525 7
The application of primary organoid cultures containing epithelial and mesenchymal elements to cancer modeling holds promise for combining the accurate multilineage differentiation and physiology of in vivo systems with the facile in vitro manipulation of transformed cell lines. Here we used a single air-liquid interface culture method without modification to engineer oncogenic mutations into primary epithelial and mesenchymal organoids from mouse colon, stomach and pancreas. Pancreatic and gast ...[more]