Ontology highlight
ABSTRACT:
SUBMITTER: Fu X
PROVIDER: S-EPMC4087297 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Fu Xinhua X Wang Wei W Hao Jianhua J Zhu Xianglin X Sun Mi M
BioMed research international 20140618
The catalase from marine bacterium Acinetobacter sp. YS0810 (YS0810CAT) was purified and characterized. Consecutive steps were used to achieve the purified enzyme as follows: ethanol precipitation, DEAE Sepharose ion exchange, Superdex 200 gel filtration, and Resource Q ion exchange. The active enzyme consisted of four identical subunits of 57.256 kDa. It showed a Soret peak at 405 nm, indicating the presence of iron protoporphyrin IX. The catalase was not apparently reduced by sodium dithionite ...[more]