Ontology highlight
ABSTRACT:
SUBMITTER: Gold VA
PROVIDER: S-EPMC4090714 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Gold Vicki A M VA Ieva Raffaele R Walter Andreas A Pfanner Nikolaus N van der Laan Martin M Kühlbrandt Werner W
Nature communications 20140619
Unravelling the structural organization of membrane protein machines in their active state and native lipid environment is a major challenge in modern cell biology research. Here we develop the STAMP (Specifically TArgeted Membrane nanoParticle) technique as a strategy to localize protein complexes in situ by electron cryotomography (cryo-ET). STAMP selects active membrane protein complexes and marks them with quantum dots. Taking advantage of new electron detector technology that is currently r ...[more]