Ontology highlight
ABSTRACT:
SUBMITTER: Kast DJ
PROVIDER: S-EPMC4091835 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Nature structural & molecular biology 20140302 4
The Rho family GTPase effector IRSp53 has essential roles in filopodia formation and neuronal development, but its regulatory mechanism is poorly understood. IRSp53 contains a membrane-binding BAR domain followed by an unconventional CRIB motif that overlaps with a proline-rich region (CRIB-PR) and an SH3 domain that recruits actin cytoskeleton effectors. Using a fluorescence reporter assay, we show that human IRSp53 adopts a closed inactive conformation that opens synergistically with the bindi ...[more]