Ontology highlight
ABSTRACT:
SUBMITTER: Rossi G
PROVIDER: S-EPMC7041683 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Rossi Guendalina G Lepore Dante D Kenner Lillian L Czuchra Alexander B AB Plooster Melissa M Frost Adam A Munson Mary M Brennwald Patrick P
The Journal of cell biology 20200201 2
The exocyst complex plays a critical role in determining both temporal and spatial dynamics of exocytic vesicle tethering and fusion with the plasma membrane. However, the mechanism by which the exocyst functions and how it is regulated remain poorly understood. Here we describe a novel biochemical assay for the examination of exocyst function in vesicle tethering. Importantly, the assay is stimulated by gain-of-function mutations in the Exo70 component of the exocyst, selected for their ability ...[more]