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Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.

ABSTRACT: An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

SUBMITTER: Bosse JT

PROVIDER: S-EPMC4097740 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.

Bossé Janine T JT Li Yanwen Y Angen Øystein Ø Weinert Lucy A LA Chaudhuri Roy R RR Holden Matthew T MT Williamson Susanna M SM Maskell Duncan J DJ Tucker Alexander W AW Wren Brendan W BW Rycroft Andrew N AN Langford Paul R PR

Journal of clinical microbiology 20140423 7

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range ...[more]

PMID: 24759717

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