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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.


ABSTRACT: Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

SUBMITTER: Bosse JT 

PROVIDER: S-EPMC6008488 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.

Bossé Janine T JT   Li Yanwen Y   Fernandez Crespo Roberto R   Lacouture Sonia S   Gottschalk Marcelo M   Sárközi Rita R   Fodor László L   Casas Amoribieta Maria M   Angen Øystein Ø   Nedbalcova Katerina K   Holden Matthew T G MTG   Maskell Duncan J DJ   Tucker Alexander W AW   Wren Brendan W BW   Rycroft Andrew N AN   Langford Paul R PR  

Veterinary microbiology 20180520


Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleurop  ...[more]

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