Ontology highlight
ABSTRACT:
SUBMITTER: Jorda J
PROVIDER: S-EPMC4101507 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Jordà Joel J Rojas Hugo Cueto HC Carnicer Marc M Wahl Aljoscha A Ferrer Pau P Albiol Joan J
Metabolites 20140505 2
Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (Rol) on P. pastoris central carbon metabolism. Higher oxygen uptake and CO2 production rates and slightly reduced biomass yield suggest an increased energy demand for the producing strain. This observatio ...[more]