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TRF2, but not TBP, mediates the transcription of ribosomal protein genes.

ABSTRACT: The TCT core promoter element is present in most ribosomal protein (RP) genes in Drosophila and humans. Here we show that TBP (TATA box-binding protein)-related factor TRF2, but not TBP, is required for transcription of the TCT-dependent RP genes. In cells, TCT-dependent transcription, but not TATA-dependent transcription, increases or decreases upon overexpression or depletion of TRF2. In vitro, purified TRF2 activates TCT but not TATA promoters. ChIP-seq (chromatin immunoprecipitation [ChIP] combined with deep sequencing) experiments revealed the preferential localization of TRF2 at TCT versus TATA promoters. Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems.

SUBMITTER: Wang YL

PROVIDER: S-EPMC4102762 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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TRF2, but not TBP, mediates the transcription of ribosomal protein genes.

Wang Yuan-Liang YL Duttke Sascha H C SH Chen Kai K Johnston Jeff J Kassavetis George A GA Zeitlinger Julia J Kadonaga James T JT

Genes & development 20140623 14

The TCT core promoter element is present in most ribosomal protein (RP) genes in Drosophila and humans. Here we show that TBP (TATA box-binding protein)-related factor TRF2, but not TBP, is required for transcription of the TCT-dependent RP genes. In cells, TCT-dependent transcription, but not TATA-dependent transcription, increases or decreases upon overexpression or depletion of TRF2. In vitro, purified TRF2 activates TCT but not TATA promoters. ChIP-seq (chromatin immunoprecipitation [ChIP] c ...[more]

PMID: 24958592

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