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Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae).


ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite primers were identified and characterized in Acca sellowiana in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. • METHODS AND RESULTS:A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)-enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0. • CONCLUSIONS:These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in A. sellowiana.

SUBMITTER: Klabunde GH 

PROVIDER: S-EPMC4103439 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae).

Klabunde Gustavo H F GH   Olkoski Denise D   Vilperte Vinicius V   Zucchi Maria I MI   Nodari Rubens O RO  

Applications in plant sciences 20140604 6


<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite primers were identified and characterized in Acca sellowiana in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. •<h4>Methods and results</h4>A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)-enriched genomic library. The primers amplified di-  ...[more]

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