Development and characterization of 10 microsatellite markers in Sagina nodosa (Caryophyllaceae).
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ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:We developed 10 novel microsatellite loci for Sagina nodosa, a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. • METHODS AND RESULTS:We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. • CONCLUSIONS:These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations.
SUBMITTER: Singh T
PROVIDER: S-EPMC4123380 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
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