Unknown

Dataset Information

0

Development and characterization of 10 microsatellite markers in Sagina nodosa (Caryophyllaceae).


ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:We developed 10 novel microsatellite loci for Sagina nodosa, a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. • METHODS AND RESULTS:We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. • CONCLUSIONS:These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations.

SUBMITTER: Singh T 

PROVIDER: S-EPMC4123380 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Development and characterization of 10 microsatellite markers in Sagina nodosa (Caryophyllaceae).

Singh Tarjinder T   Edwards Joan J   Maroja Luana S LS  

Applications in plant sciences 20140107 1


<h4>Unlabelled</h4><h4>Premise of the study</h4>We developed 10 novel microsatellite loci for Sagina nodosa, a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. •<h4>Methods and results</h4>We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polym  ...[more]

Similar Datasets

| S-EPMC6325733 | biostudies-literature
| S-EPMC6303153 | biostudies-literature
| S-EPMC4578374 | biostudies-literature
| S-EPMC5238704 | biostudies-literature
| S-EPMC4103439 | biostudies-literature
| S-EPMC7073324 | biostudies-literature
| S-EPMC4105283 | biostudies-literature
| S-EPMC4105279 | biostudies-literature
| S-EPMC4332145 | biostudies-literature
| S-EPMC4467762 | biostudies-literature