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Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • METHODS AND RESULTS:Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1-10 alleles per population. • CONCLUSIONS:These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata.

SUBMITTER: Van Etten ML

PROVIDER: S-EPMC4103472 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae).

Van Etten Megan L ML Robertson Alastair W AW Tate Jennifer A JA

Applications in plant sciences 20131001 10

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. •<h4>Methods and results</h4>Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populat ...[more]

PMID: 25202492

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