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Isolation of fucosyltransferase-producing bacteria from marine environments.


ABSTRACT: Fucose-containing oligosaccharides on the cell surface of some pathogenic bacteria are thought to be important for host-microbe interactions and to play a major role in the pathogenicity of bacterial pathogens. Here, we screened marine bacteria for glycosyltransferases using two methods: a one-pot glycosyltransferase assay method and a lectin-staining method. Using this approach, we isolated marine bacteria with fucosyltransferase activity. There have been no previous reports of marine bacteria producing fucosyltransferase. This paper thus represents the first report of fucosyltransferase-producing marine bacteria.

SUBMITTER: Kajiwara H 

PROVIDER: S-EPMC4103564 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Isolation of fucosyltransferase-producing bacteria from marine environments.

Kajiwara Hitomi H   Toda Munetoyo M   Mine Toshiki T   Nakada Hiroshi H   Yamamoto Takeshi T  

Microbes and environments 20121026 4


Fucose-containing oligosaccharides on the cell surface of some pathogenic bacteria are thought to be important for host-microbe interactions and to play a major role in the pathogenicity of bacterial pathogens. Here, we screened marine bacteria for glycosyltransferases using two methods: a one-pot glycosyltransferase assay method and a lectin-staining method. Using this approach, we isolated marine bacteria with fucosyltransferase activity. There have been no previous reports of marine bacteria  ...[more]

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