Ontology highlight
ABSTRACT:
SUBMITTER: Currie JD
PROVIDER: S-EPMC4104172 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Currie Joshua D JD Rogers Stephen L SL
Nature protocols 20110929 10
Cultured Drosophila melanogaster S2 and S2R+ cell lines have become important tools for uncovering fundamental aspects of cell biology as well as for gene discovery. Despite their utility, these cell lines are nonmotile and cannot build polarized structures or cell-cell contacts. Here we outline a previously isolated, but uncharacterized, Drosophila cell line named Dm-D17-c3 (or D17). These cells spread and migrate in culture, form cell-cell junctions and are susceptible to RNA interference (RNA ...[more]