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Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species.

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. • METHODS AND RESULTS:Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica. • CONCLUSIONS:The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.

SUBMITTER: Martins AR 

PROVIDER: S-EPMC4105028 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species.

Martins Aline R AR   Abreu Aluana G AG   Bajay Miklos M MM   Villela Priscilla M S PM   Batista Carlos E A CE   Monteiro Mariza M   Alves-Pereira Alessandro A   Figueira Glyn M GM   Pinheiro José B JB   Appezzato-da-Glória Beatriz B   Zucchi Maria I MI  

Applications in plant sciences 20130516 6

<h4>Unlabelled</h4><h4>Premise of the study</h4>A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. •<h4>Methods and results</h4>Using a protocol for genomic library enrichment, primer pairs were developed fo  ...[more]

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