Unknown

Dataset Information

0

Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species.

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. • METHODS AND RESULTS:Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica. • CONCLUSIONS:The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.

SUBMITTER: Martins AR 

PROVIDER: S-EPMC4105028 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species.

Martins Aline R AR   Abreu Aluana G AG   Bajay Miklos M MM   Villela Priscilla M S PM   Batista Carlos E A CE   Monteiro Mariza M   Alves-Pereira Alessandro A   Figueira Glyn M GM   Pinheiro José B JB   Appezzato-da-Glória Beatriz B   Zucchi Maria I MI  

Applications in plant sciences 20130516 6

<h4>Unlabelled</h4><h4>Premise of the study</h4>A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. •<h4>Methods and results</h4>Using a protocol for genomic library enrichment, primer pairs were developed fo  ...[more]

Similar Datasets

Unknown Isolation and characterization of microsatellite loci for Smilax sieboldii (Smilacaceae).
| S-EPMC5357126 | biostudies-literature
Unknown Characterization of polymorphic microsatellite loci for North American common greenbrier, <i>Smilax rotundifolia</i> (Smilacaceae).
| S-EPMC6025819 | biostudies-literature
Unknown Development of microsatellite loci in Mediterranean sarsaparilla (Smilax aspera; Smilacaceae) using transcriptome data.
| S-EPMC5400434 | biostudies-literature
Unknown Development of microsatellite markers in Ilex kaushue (Aquifoliaceae), a medicinal plant species.
| S-EPMC4542944 | biostudies-literature
Unknown Smilax weniae, a New Species of Smilacaceae from Limestone Areas Bordering Guizhou and Guangxi, China.
| S-EPMC9028124 | biostudies-literature
Unknown Characterization of 13 microsatellite markers for Diuris basaltica (Orchidaceae) and related species.
| S-EPMC4104714 | biostudies-literature
Unknown Characterization of microsatellite markers for <i>Moricandia moricandioides</i> (Brassicaceae) and related species.
| S-EPMC6110244 | biostudies-literature
Unknown Isolation and characterization of eleven polymorphic microsatellite loci for the valuable medicinal plant Dendrobium huoshanense and cross-species amplification.
| S-EPMC3546720 | biostudies-literature
Unknown Characterization of 15 nuclear microsatellite markers for <i>Afzelia africana</i> (Fabaceae) and related species.
| S-EPMC6526647 | biostudies-literature
Unknown Characterization of microsatellite markers for the endangered <i>Daphne rodriguezii</i> (Thymelaeaceae) and related species.
| S-EPMC6636617 | biostudies-literature