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Development of microsatellite markers in Ilex kaushue (Aquifoliaceae), a medicinal plant species.

ABSTRACT: PREMISE OF THE STUDY:Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity. METHODS AND RESULTS:Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. The number of alleles per locus ranged from one to nine across 22 individuals from both cultivated and wild populations. The observed and expected heterozygosity in these two populations ranged from 0.000 to 1.000 and from 0.000 to 0.785, respectively. CONCLUSIONS:These markers will be useful in studies on genetic diversity of I. kaushue.

SUBMITTER: Qin LF

PROVIDER: S-EPMC4542944 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Development of microsatellite markers in Ilex kaushue (Aquifoliaceae), a medicinal plant species.

Qin Lan-Fang LF Qu Xin-Cheng XC Hu Gang G Huang Yun-Feng YF Zhang Qi-Wei QW

Applications in plant sciences 20150810 8

<h4>Premise of the study</h4>Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity.<h4>Methods and results</h4>Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. The number of alleles per locus ranged from one to nine across 22 individuals from both cul ...[more]

PMID: 26312197

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