Ontology highlight
ABSTRACT:
SUBMITTER: Larson MH
PROVIDER: S-EPMC4108260 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Larson Matthew H MH Mooney Rachel A RA Peters Jason M JM Windgassen Tricia T Nayak Dhananjaya D Gross Carol A CA Block Steven M SM Greenleaf William J WJ Landick Robert R Weissman Jonathan S JS
Science (New York, N.Y.) 20140501 6187
Transcription by RNA polymerase (RNAP) is interrupted by pauses that play diverse regulatory roles. Although individual pauses have been studied in vitro, the determinants of pauses in vivo and their distribution throughout the bacterial genome remain unknown. Using nascent transcript sequencing, we identified a 16-nucleotide consensus pause sequence in Escherichia coli that accounts for known regulatory pause sites as well as ~20,000 new in vivo pause sites. In vitro single-molecule and ensembl ...[more]