Dataset Information

Potential Use of Rice Field Cyanobacterium Nostoc muscorum in the Evaluation of Butachlor Induced Toxicity and their Degradation.

ABSTRACT: In the present study, butachlor (5, 10, 20, 40 and 80 ppm) induced toxicity in Nostoc muscorum and their degradation was evaluated. The dose of butachlor dependent decreased in the cell survival and growth of N. muscorum was noticed. Scanning electron microscopy revealed the adverse impact on the cell size and shapes. Low concentrations of butachlor (10 and 20 ppm) induced the over expression of a polypeptides of 31.0 K Da and 42.7 K Da, respectively which could be responsible for developing resistance in the organism up to certain level. Further, the degradation product of butachlor as a result of metabolic activities of N. muscorum, identified by GC-MS analysis includes phenols and benzene dicarboxylic acid indicating the utilization of herbicide during active growth.

SUBMITTER: Anees S

PROVIDER: S-EPMC4110428 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Potential Use of Rice Field Cyanobacterium Nostoc muscorum in the Evaluation of Butachlor Induced Toxicity and their Degradation.

Anees Sumaiya S Suhail Shazia S Pathak Neelam N Zeeshan Mohd M

Bioinformation 20140630 6

In the present study, butachlor (5, 10, 20, 40 and 80 ppm) induced toxicity in Nostoc muscorum and their degradation was evaluated. The dose of butachlor dependent decreased in the cell survival and growth of N. muscorum was noticed. Scanning electron microscopy revealed the adverse impact on the cell size and shapes. Low concentrations of butachlor (10 and 20 ppm) induced the over expression of a polypeptides of 31.0 K Da and 42.7 K Da, respectively which could be responsible for developing res ...[more]

PMID: 25097380

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Project description:PurposeThe biological synthesis of nanoparticles (NPs) has become a new methodology for the eco-friendly production of NPs with high scalability and biocompatibility. Cyanobacteria are one of the most widespread microorganisms on Earth and have been proven to be successful biofactories for synthesizing NPs. It is challenging to discover new microalgae with the potential to synthesize NPs of small size with high stability.MethodsNostoc muscorum Lukesova 2/91 was isolated, purified, and identified morphologically and genetically using microscopy and DNA sequencing. Volatile biomolecules in aqueous algal extracts were assessed using gas chromatography-mass spectroscopy (GC-MS).ResultsData showed that the main biomolecules were fatty acids and their esters, followed by secondary metabolites. Algal extract was used to convert silver nitrate (AgNO3) into silver NPs under various optimized parameters. 1 mM of AgNO3, 1:1 (V/V ratio of algal extract to AgNO3), 25 °C, under light illumination, for 24 h, at pH 7.4 were the optimum conditions for NP production (Nos@AgNPs). Nos@AgNPs were characterized using UV-VIS spectroscopy, FTIR, TEM, SEM, EDx, mapping, and a Zetasizer. The wavelength of Nos@AgNPs was 401.4 nm and their shapes were cubic to oval, with an average diameter of 11.8 ± 0.5 nm. FTIR spectroscopy revealed that proteins/polysaccharides could be the main reductants, whereas these molecules and/or fatty acids could be stabilizers for NP synthesis. Nos@AgNPs (86.15%) was silver and had a hydrodynamic diameter of 10.7 nm with a potential charge of -19.7 mV. Antiproliferative and antimicrobial activities of Nos@AgNPs were evaluated. Nos@AgNPs exhibited significant inhibitory activity against lung, colon, and breast cancer cells and considerable biocidal activity against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa.ConclusionN. muscorum Lukesova 2/91 is an excellent source for the biofabrication of small and stable AgNPs with potent inhibitory effects against cancer and bacterial cells.

Project description:Ice-binding proteins (IBPs) have been identified in numerous polar algae and bacteria, but so far not in any cyanobacteria, despite the abundance of cyanobacteria in polar regions. We previously reported strong IBP activity associated with an Antarctic Nostoc species. In this study, to identify the proteins responsible, as well as elucidate their origin, we sequenced the DNA of an environmental sample of this species, designated Nostoc sp. HG1, and its bacterial community and attempted to identify IBPs by looking for known IBPs in the metagenome and by looking for novel IBPs by tandem mass spectrometry (MS/MS) proteomics analyses of ice affinity-purified proteins. The metagenome contained over 116 DUF3494-type IBP genes, the most common type of IBP identified so far. One of the IBPs could be confidently assigned to Nostoc, while the others could be attributed to diverse bacteria, which, surprisingly, accounted for the great majority of the metagenome. Recombinant Nostoc IBPs (nIBPs) had strong ice-structuring activities, and their circular dichroism spectra were consistent with the secondary structure of a DUF3494-type IBP. nIBP is unusual in that it is the only IBP identified so far to have a PEP (amino acid motif) C-terminal signal, a signal that has been associated with anchoring to the outer cell membrane. These results suggest that the observed IBP activity of Nostoc sp. HG1 was due to a combination of endogenous and exogenous IBPs. Amino acid and nucleotide sequence analyses of nIBP raise the possibility that it was acquired from a planctomycete.IMPORTANCE The horizontal transfer of genes encoding ice-binding proteins (IBPs), proteins that confer freeze-thaw tolerance, has allowed many microorganisms to expand their ranges into polar regions. One group of microorganisms for which nothing is known about its IBPs is cyanobacteria. In this study, we identified a cyanobacterial IBP and showed that it was likely acquired from another bacterium, probably a planctomycete. We also showed that a consortium of IBP-producing bacteria living with the Nostoc contribute to its IBP activity.

Dataset Information

Potential Use of Rice Field Cyanobacterium Nostoc muscorum in the Evaluation of Butachlor Induced Toxicity and their Degradation.

Publications

Potential Use of Rice Field Cyanobacterium Nostoc muscorum in the Evaluation of Butachlor Induced Toxicity and their Degradation.

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