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Biosorption and equilibrium isotherms study of cadmium removal by Nostoc muscorum Meg 1: morphological, physiological and biochemical alterations.

ABSTRACT: Rice fields of Meghalaya especially in the coal mining belt receive water contaminated by effluents from mines that are known to carry harmful heavy metal ions such as Cu, Fe, Zn, Ni, Cd, As, Pb, Cr, etc. Cd exposure was analyzed in the cyanobacterium Nostoc muscorum Meg 1 isolated from a contaminated rice field in Sohra, Meghalaya, India. Toxicity study established 0.5 ppm on day 3 to be the LD50. At LD50 chlorophyll a and total protein concentration was reduced by 50.9 and 52.5%, while nitrogenase and glutamine synthetase activities were inhibited by 40.8 and 38.4%. EDX and FTIR analyses confirmed Cd binding and participation of hydroxyl, carbonyl, carboxyl and phosphate groups in biosorption of Cd onto the cell surfaces. SEM study established morphological changes. At pH 8.0 and temperature 25 ± 2 °C, the cyanobacterium removed 92% Cd within 24 h. Of this, 91% Cd was adsorbed on the cell surface while 4% was internally accumulated. The energy required for internal accumulation of Cd was partly provided in the form of ATP synthesized during active photosynthesis. The Langmuir isotherm was found best fitted with a R 2 value 0.98 when compared to Freundlich and Temkin adsorption isotherms. The maximum sorption capacity, Q max, of the organism was 71.4 mg of Cd per g of biomass. R L value of 0.29 indicated favorable interaction between cyanobacterial biomass and Cd. The adsorption intensity, n value 7.69 g/L obtained from Freundlich isotherm showed that the organism possessed high Cd sorption capacity.

SUBMITTER: Ahad RIA

PROVIDER: S-EPMC5449281 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Biosorption and equilibrium isotherms study of cadmium removal by Nostoc muscorum Meg 1: morphological, physiological and biochemical alterations.

Ahad Rabbul Ibne A RIA Goswami Smita S Syiem Mayashree B MB

3 Biotech 20170530 2

Rice fields of Meghalaya especially in the coal mining belt receive water contaminated by effluents from mines that are known to carry harmful heavy metal ions such as Cu, Fe, Zn, Ni, Cd, As, Pb, Cr, etc. Cd exposure was analyzed in the cyanobacterium Nostoc muscorum Meg 1 isolated from a contaminated rice field in Sohra, Meghalaya, India. Toxicity study established 0.5 ppm on day 3 to be the LD<sub>50</sub>. At LD<sub>50</sub> chlorophyll a and total protein concentration was reduced by 50.9 an ...[more]

PMID: 28560644

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Project description:PurposeThe biological synthesis of nanoparticles (NPs) has become a new methodology for the eco-friendly production of NPs with high scalability and biocompatibility. Cyanobacteria are one of the most widespread microorganisms on Earth and have been proven to be successful biofactories for synthesizing NPs. It is challenging to discover new microalgae with the potential to synthesize NPs of small size with high stability.MethodsNostoc muscorum Lukesova 2/91 was isolated, purified, and identified morphologically and genetically using microscopy and DNA sequencing. Volatile biomolecules in aqueous algal extracts were assessed using gas chromatography-mass spectroscopy (GC-MS).ResultsData showed that the main biomolecules were fatty acids and their esters, followed by secondary metabolites. Algal extract was used to convert silver nitrate (AgNO3) into silver NPs under various optimized parameters. 1 mM of AgNO3, 1:1 (V/V ratio of algal extract to AgNO3), 25 °C, under light illumination, for 24 h, at pH 7.4 were the optimum conditions for NP production (Nos@AgNPs). Nos@AgNPs were characterized using UV-VIS spectroscopy, FTIR, TEM, SEM, EDx, mapping, and a Zetasizer. The wavelength of Nos@AgNPs was 401.4 nm and their shapes were cubic to oval, with an average diameter of 11.8 ± 0.5 nm. FTIR spectroscopy revealed that proteins/polysaccharides could be the main reductants, whereas these molecules and/or fatty acids could be stabilizers for NP synthesis. Nos@AgNPs (86.15%) was silver and had a hydrodynamic diameter of 10.7 nm with a potential charge of -19.7 mV. Antiproliferative and antimicrobial activities of Nos@AgNPs were evaluated. Nos@AgNPs exhibited significant inhibitory activity against lung, colon, and breast cancer cells and considerable biocidal activity against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa.ConclusionN. muscorum Lukesova 2/91 is an excellent source for the biofabrication of small and stable AgNPs with potent inhibitory effects against cancer and bacterial cells.

Project description:In comparison to physicochemical and chemical methods, microbial dye biosorption is regarded as an eco-effective and economically viable alternative and is a widely applied method due to its high efficiency and compatibility with the environment. Therefore, the idea of this study is to clarify to what extent the viable cells and the dry biomass of Pseudomonas alcaliphila NEWG-2 can improve the biosorption of methylene blue (MB) from a synthetic wastewater sample. The array of Taguchi paradigm has been conducted to ascertain five variables affecting the biosorption of MB by broth forms of P. alcaliphila NEWG. The data of MB biosorption were familiar to the predicted ones, indicating the precision of the Taguchi model's prediction. The maximum biosorption of MB (87.14%) was achieved at pH 8, after 60 h, in a medium containing 15 mg/ml MB, 2.5% glucose, and 2% peptone, with sorting the highest signal-to-noise ratio (38.80). FTIR spectra detected various functional groups (primary alcohol, α, β-unsaturated ester, symmetric NH2 bending, and strong C-O stretching) on the bacterial cell wall that participated in the biosorption of MB. Furthermore, the spectacular MB biosorption ability was validated by equilibrium isotherms and kinetic studies (the dry biomass form), which were derived from the Langmuir model (qmax = 68.827 mg/g). The equilibrium time was achieved in about 60 min, with 70.5% of MB removal. The biosorption kinetic profile might be adequately represented by pseudo-second order and Elovich models. The changes in the bacterial cells before and after the biosorption of MB were characterized using a scanning electron microscope. As realized from the aforementioned data, the bacterium is a talented, effective, eco-friendly, and low-cost bio-sorbent for the decolorization and remedy of an industrial effluent containing MB from an aqueous environment. The current outcomes in the biosorption of MB molecules promote the use of the bacterial strain as viable cells and/or dry biomass in ecosystem restoration, environmental cleanup, and bioremediation studies.

Dataset Information

Biosorption and equilibrium isotherms study of cadmium removal by Nostoc muscorum Meg 1: morphological, physiological and biochemical alterations.

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Biosorption and equilibrium isotherms study of cadmium removal by Nostoc muscorum Meg 1: morphological, physiological and biochemical alterations.

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