Relevance of α-defensins (HNP1-3) and defensin β-1 in diabetes.

ABSTRACT:

Aim

To investigate the genetic background of human defensin expression in type 1 and 2 diabetes.

Methods

Associations between DEFA1/DEFA3 gene copy number polymorphism and diabetes as well as between the promoter polymorphisms of DEFB1 and diabetes were studied. The copy number variation of the DEFA1/DEFA3 genes was determined in 257 diabetic patients (117 patients with type 1 and 140 with type 2 diabetes). The control group consisted of 221 age- and gender-matched healthy blood donors. The cumulative copy numbers of the DEFA1/DEFA3 genes were detected by using quantitative PCR analysis. To evaluate the HNP 1-3 (human neutrophil peptide 1-3 or α-defensin) levels in the circulation, plasma HNP 1-3 concentrations were measured by ELISA. The expression of DEFA1/A3 in peripheral

SUBMITTER: Nemeth BC 

PROVIDER: S-EPMC4112898 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature