Dataset Information

Human Salivary Neutrophil Defensins (Alpha-Defensins)

ABSTRACT: In the HPLC high-resolution ESI-MS profiles of both Predominantly Antibody Deficiency syndromes subjects and healthy controls saliva samples, four proteins, with exp. monoisotopic ion [M+H]+ at 3440.54 ± 0.06 m/z, 3369.50± 0.06 m/z, 3484.51 ± 0.06 m/z and 3707.77 ± 0.06, eluting between 24.5-26.6 minutes, were detected. The monoisotopic mass values and the manual inspection of the high-resolution MS/MS spectra of the ions at [M+5H]+5 at 689.31, 675.10, 698.11 and 742.76 m/z, and of the ions at [M+4H]+4 at 843.63, 872.39 m/z allowed to establish that the four proteins were alpha defensins 1, 2, 3 and 4.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Barbara Manconi

LAB HEAD: Barbara Manconi

PROVIDER: PXD012688 | Pride | 2020-01-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CSM033_Roma_HCD_07102015.raw	Raw
	ManualAnnotation_alpha_defensins_TopDown.xlsx	Xlsx
	SM024_Roma_HCD_250915.raw	Raw
	SM028_Roma_HCD_250915.raw	Raw

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Publications

RP-HPLC-ESI-IT Mass Spectrometry Reveals Significant Variations of the Human Salivary Protein Profile Associated with Predominantly Antibody Deficiencies.

Contini Cristina C Firinu Davide D Serrao Simone S Manconi Barbara B Olianas Alessandra A Cinetto Francesco F Cossu Fausto F Castagnola Massimo M Messana Irene I Del Giacco Stefano S Cabras Tiziana T

Journal of clinical immunology 20200108 2

<h4>Purpose</h4>Present study is designed to discover potential salivary biomarkers associated with predominantly antibody deficiencies, which include a large spectrum of disorders sharing failure of antibody production, and B cell defects resulting in recurrent infections, autoimmune and inflammatory manifestations, and tumor susceptibility. Understanding and clinical classification of these syndromes is still challenging.<h4>Methods</h4>We carried out a study of human saliva based on liquid ch ...[more]

PMID: 31916122

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