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Environmentally responsive histidine-carboxylate zipper formation between proteins and nanoparticles.

ABSTRACT: Interfacing synthetic materials with biomacromolecules provides new systems for biological applications. We report the creation of a reversible multivalent supramolecular "zipper" recognition motif between gold nanoparticles and proteins. In this assembly, carboxylate-functionalized nanoparticles interact strongly with oligohistidine tags. This interaction can be tuned through His-tag length, and offers unique binding profiles based on the pH and electrolyte concentration of the medium.

SUBMITTER: Mout R

PROVIDER: S-EPMC4113908 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Environmentally responsive histidine-carboxylate zipper formation between proteins and nanoparticles.

Mout Rubul R Tonga Gulen Yesilbag GY Ray Moumita M Moyano Daniel F DF Xing Yuqing Y Rotello Vincent M VM

Nanoscale 20140801 15

Interfacing synthetic materials with biomacromolecules provides new systems for biological applications. We report the creation of a reversible multivalent supramolecular "zipper" recognition motif between gold nanoparticles and proteins. In this assembly, carboxylate-functionalized nanoparticles interact strongly with oligohistidine tags. This interaction can be tuned through His-tag length, and offers unique binding profiles based on the pH and electrolyte concentration of the medium. ...[more]

PMID: 24960536

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