Ontology highlight
ABSTRACT:
SUBMITTER: Simon AB
PROVIDER: S-EPMC4114222 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Simon Arlyne B AB Frampton John P JP Huang Nien-Tsu NT Kurabayashi Katsuo K Paczesny Sophie S Takayama Shuichi S
Technology 20140601 2
Quantitative measurement of protein biomarkers is critical for biomarker validation and early disease detection. Current multiplex immunoassays are time consuming costly and can suffer from low accuracy. For example, multiplex ELISAs require multiple, tedious, washing and blocking steps. Moreover, they suffer from nonspecific antibody cross-reactions, leading to high background and false-positive signals. Here, we show that co-localizing antibody-bead pairs in an aqueous two-phase system (ATPS) ...[more]