Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC4115501 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Liu Yuchen Y Nakamura Akiyoshi A Nakazawa Yuto Y Asano Nozomi N Ford Kara A KA Hohn Michael J MJ Tanaka Isao I Yao Min M Söll Dieter D
Proceedings of the National Academy of Sciences of the United States of America 20140707 29
Methanogenic archaea lack cysteinyl-tRNA synthetase; they synthesize Cys-tRNA and cysteine in a tRNA-dependent manner. Two enzymes are required: Phosphoseryl-tRNA synthetase (SepRS) forms phosphoseryl-tRNA(Cys) (Sep-tRNA(Cys)), which is converted to Cys-tRNA(Cys) by Sep-tRNA:Cys-tRNA synthase (SepCysS). This represents the ancestral pathway of Cys biosynthesis and coding in archaea. Here we report a translation factor, SepCysE, essential for methanococcal Cys biosynthesis; its deletion in Methan ...[more]