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Neurospora crassa 1,3-?-glucan synthase, AGS-1, is required for cell wall biosynthesis during macroconidia development.


ABSTRACT: The Neurospora crassa genome encodes two 1,3-?-glucan synthases. One of these 1,3-?-glucan synthase genes, ags-1, was shown to be required for the synthesis of 1,3-?-glucan in the aerial hyphae and macroconidia cell walls. 1,3-?-Glucan was found in the conidia cell wall, but was absent from the vegetative hyphae cell wall. Deletion of ags-1 affected conidial development. ?ags-1 produced only 5?% as many conidia as the WT and most of the conidia produced by ?ags-1 were not viable. The ags-1 upstream regulatory elements were shown to direct cell-type-specific expression of red fluorescent protein in conidia and aerial hyphae. A haemagglutinin-tagged AGS-1 was found to be expressed in aerial hyphae and conidia. The research showed that 1,3-?-glucan is an aerial hyphae and conidia cell wall component, and is required for normal conidial differentiation.

SUBMITTER: Fu C 

PROVIDER: S-EPMC4117219 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Neurospora crassa 1,3-α-glucan synthase, AGS-1, is required for cell wall biosynthesis during macroconidia development.

Fu Ci C   Tanaka Asuma A   Free Stephen J SJ  

Microbiology (Reading, England) 20140520 Pt 8


The Neurospora crassa genome encodes two 1,3-α-glucan synthases. One of these 1,3-α-glucan synthase genes, ags-1, was shown to be required for the synthesis of 1,3-α-glucan in the aerial hyphae and macroconidia cell walls. 1,3-α-Glucan was found in the conidia cell wall, but was absent from the vegetative hyphae cell wall. Deletion of ags-1 affected conidial development. Δags-1 produced only 5 % as many conidia as the WT and most of the conidia produced by Δags-1 were not viable. The ags-1 upstr  ...[more]

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