Unknown

Dataset Information

0

Cloning and characterization of a Neurospora crassa gene required for (1,3) beta-glucan synthase activity and cell wall formation.


ABSTRACT: The glucan synthase 1 gene (gs-1) is required for (1,3) beta-glucan synthase activity [E.C. 2.4.1.34; UDP glucose:1,3-beta-D-glucan 3-beta-D-glucosyltransferase] and for cell wall formation. The gs-1 gene was cloned by functional complementation of the cell-wall-less defect of the (1,3) beta-glucan synthase-deficient mutant, TM1, by using a genomic Neurospora crassa cosmid library. A 2568-nucleotide gs-1 cDNA sequence revealed a 532-amino acid open reading frame encoding a polypeptide of 59 kDa. The predicted gs-1 gene product has no obvious signal peptide cleavage sites or transmembrane domains. A gs-1 null mutant is defective for cell wall formation and (1,3) beta-glucan synthase activity. The predicted GS-1 protein is weakly homologous to a putative Saccharomyces cerevisiae transcriptional regulatory protein.

SUBMITTER: Enderlin CS 

PROVIDER: S-EPMC44840 | biostudies-other | 1994 Sep

REPOSITORIES: biostudies-other

altmetric image

Publications

Cloning and characterization of a Neurospora crassa gene required for (1,3) beta-glucan synthase activity and cell wall formation.

Enderlin C S CS   Selitrennikoff C P CP  

Proceedings of the National Academy of Sciences of the United States of America 19940901 20


The glucan synthase 1 gene (gs-1) is required for (1,3) beta-glucan synthase activity [E.C. 2.4.1.34; UDP glucose:1,3-beta-D-glucan 3-beta-D-glucosyltransferase] and for cell wall formation. The gs-1 gene was cloned by functional complementation of the cell-wall-less defect of the (1,3) beta-glucan synthase-deficient mutant, TM1, by using a genomic Neurospora crassa cosmid library. A 2568-nucleotide gs-1 cDNA sequence revealed a 532-amino acid open reading frame encoding a polypeptide of 59 kDa.  ...[more]

Similar Datasets

| S-EPMC4117219 | biostudies-literature
| S-EPMC141170 | biostudies-literature
| S-EPMC4112233 | biostudies-literature
| S-EPMC10499315 | biostudies-literature
| S-EPMC3812973 | biostudies-literature
| S-EPMC7015794 | biostudies-literature
| S-EPMC7144292 | biostudies-literature
| S-EPMC7544195 | biostudies-literature
| S-EPMC1489279 | biostudies-literature
| S-EPMC3133421 | biostudies-literature