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Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.

ABSTRACT: We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.

SUBMITTER: Smallwood SA

PROVIDER: S-EPMC4117646 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.

Smallwood Sébastien A SA Lee Heather J HJ Angermueller Christof C Krueger Felix F Saadeh Heba H Peat Julian J Andrews Simon R SR Stegle Oliver O Reik Wolf W Kelsey Gavin G

Nature methods 20140720 8

We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations. ...[more]

PMID: 25042786

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