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Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.

ABSTRACT: We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

SUBMITTER: Angermueller C

PROVIDER: S-EPMC4770512 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.

Angermueller Christof C Clark Stephen J SJ Lee Heather J HJ Macaulay Iain C IC Teng Mabel J MJ Hu Tim Xiaoming TX Krueger Felix F Smallwood Sebastien S Ponting Chris P CP Voet Thierry T Kelsey Gavin G Stegle Oliver O Reik Wolf W

Nature methods 20160111 3

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes. ...[more]

PMID: 26752769

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