Ontology highlight
ABSTRACT:
SUBMITTER: Hochbaum DR
PROVIDER: S-EPMC4117813 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Hochbaum Daniel R DR Zhao Yongxin Y Farhi Samouil L SL Klapoetke Nathan N Werley Christopher A CA Kapoor Vikrant V Zou Peng P Kralj Joel M JM Maclaurin Dougal D Smedemark-Margulies Niklas N Saulnier Jessica L JL Boulting Gabriella L GL Straub Christoph C Cho Yong Ku YK Melkonian Michael M Wong Gane Ka-Shu GK Harrison D Jed DJ Murthy Venkatesh N VN Sabatini Bernardo L BL Boyden Edward S ES Campbell Robert E RE Cohen Adam E AE
Nature methods 20140622 8
All-optical electrophysiology-spatially resolved simultaneous optical perturbation and measurement of membrane voltage-would open new vistas in neuroscience research. We evolved two archaerhodopsin-based voltage indicators, QuasAr1 and QuasAr2, which show improved brightness and voltage sensitivity, have microsecond response times and produce no photocurrent. We engineered a channelrhodopsin actuator, CheRiff, which shows high light sensitivity and rapid kinetics and is spectrally orthogonal to ...[more]