Absolute quantification by droplet digital PCR versus analog real-time PCR.
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ABSTRACT:
SUBMITTER: Hindson CM
PROVIDER: S-EPMC4118677 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
Absolute quantification by droplet digital PCR versus analog real-time PCR.
Hindson Christopher M CM Chevillet John R JR Briggs Hilary A HA Gallichotte Emily N EN Ruf Ingrid K IK Hindson Benjamin J BJ Vessella Robert L RL Tewari Muneesh M
Nature methods 20130901 10
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37-86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for ...[more]
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