Ontology highlight
ABSTRACT:
SUBMITTER: Cho MJ
PROVIDER: S-EPMC4120239 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Cho Michael Jeffrey MJ Lo Agnes S Y AS Mao Xuming X Nagler Arielle R AR Ellebrecht Christoph T CT Mukherjee Eric M EM Hammers Christoph M CM Choi Eun-Jung EJ Sharma Preety M PM Uduman Mohamed M Li Hong H Rux Ann H AH Farber Sara A SA Rubin Courtney B CB Kleinstein Steven H SH Sachais Bruce S BS Posner Marshall R MR Cavacini Lisa A LA Payne Aimee S AS
Nature communications 20140619
Pemphigus vulgaris (PV) is a potentially fatal blistering disease caused by autoantibodies (autoAbs) against desmoglein 3 (Dsg3). Here, we clone anti-Dsg3 antibodies (Abs) from four PV patients and identify pathogenic VH1-46 autoAbs from all four patients. Unexpectedly, VH1-46 autoAbs had relatively few replacement mutations. We reverted antibody somatic mutations to their germline sequences to determine the requirement of mutations for autoreactivity. Three of five VH1-46 germline-reverted Abs ...[more]