Unknown

Dataset Information

0

Absence of lipofuscin in motor neurons of SOD1-linked ALS mice.


ABSTRACT: Lipofuscin, or aging pigment, is accreted as red autofluorescence in the lysosomes of motor neuron cell bodies in the ventral horn of WT mice by 3 mo of age. Strikingly, in two presymptomatic ALS mouse strains transgenic for mutant human Cu/Zn superoxide dismutase (SOD1), G85R SOD1YFP and G93A SOD1, little or no lipofuscin was detected in motor neuron cell bodies. Two markers of autophagy, sequestosome 1 (SQSTM1/p62) and microtubule-associated protein 1 light chain 3 (LC3), were examined in the motor neuron cell bodies of G85R SOD1YFP mice and found to be reduced relative to WT SOD1YFP transgenic mice. To elucidate whether the autophagy/lysosome pathway was either impaired or hyperactive in motor neurons, chloroquine was administered to 3-mo-old G85R SOD1YFP mice to block lysosomal hydrolysis. After 2 wk, lipofuscin was now observed in motor neurons, and SQSTM1 and LC3 levels approached those of WT SOD1YFP mice, suggesting that the autophagy/lysosome pathway is hyperactive in motor neurons of SOD1-linked ALS mice. This seems to be mediated at least in part through the mammalian target of rapamycin complex 1 (MTORC1) pathway, because levels of Ser757-phosphorylated Unc-51-like kinase 1 (ULK1), an MTORC1 target, were greatly reduced in the G85R SOD1YFP motor neurons, correspondent to an activated state of ULK1 that initiates autophagy.

SUBMITTER: Bandyopadhyay U 

PROVIDER: S-EPMC4121794 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6327879 | biostudies-other
| S-EPMC3799799 | biostudies-literature
| S-EPMC2650191 | biostudies-literature
2012-12-04 | E-GEOD-38820 | biostudies-arrayexpress
| S-EPMC4250117 | biostudies-literature
2012-12-04 | GSE38820 | GEO
| S-EPMC2396671 | biostudies-literature
| S-EPMC4653065 | biostudies-literature
| S-EPMC3536741 | biostudies-literature
| S-EPMC4230530 | biostudies-literature