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Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.

ABSTRACT: As microfluidics has been applied extensively in many cell and biochemical applications, monitoring the related processes is an important requirement. In this work, we design and fabricate a high-throughput microfluidic device which contains 32 microchambers to perform automated parallel microfluidic operations and monitoring on an automated stage of a microscope. Images are captured at multiple spots on the device during the operations for monitoring samples in microchambers in parallel; yet the device positions may vary at different time points throughout operations as the device moves back and forth on a motorized microscopic stage. Here, we report an image-based positioning strategy to realign the chamber position before every recording of microscopic image. We fabricate alignment marks at defined locations next to the chambers in the microfluidic device as reference positions. We also develop image processing algorithms to recognize the chamber positions in real-time, followed by realigning the chambers to their preset positions in the captured images. We perform experiments to validate and characterize the device functionality and the automated realignment operation. Together, this microfluidic realignment strategy can be a platform technology to achieve precise positioning of multiple chambers for general microfluidic applications requiring long-term parallel monitoring of cell and biochemical activities.

SUBMITTER: Yip HM

PROVIDER: S-EPMC4124227 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.

Yip Hon Ming HM Li John C S JC Xie Kai K Cui Xin X Prasad Agrim A Gao Qiannan Q Leung Chi Chiu CC Lam Raymond H W RH

TheScientificWorldJournal 20140715

As microfluidics has been applied extensively in many cell and biochemical applications, monitoring the related processes is an important requirement. In this work, we design and fabricate a high-throughput microfluidic device which contains 32 microchambers to perform automated parallel microfluidic operations and monitoring on an automated stage of a microscope. Images are captured at multiple spots on the device during the operations for monitoring samples in microchambers in parallel; yet th ...[more]

PMID: 25133248

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Project description:ObjectiveTo realize the regulation of the position of corn seed planting in precision farming, an intelligent monitoring system is designed for corn seeding based on machine vision and the Genetic Algorithm-optimized Back Propagation (GABP) algorithm.MethodsBased on the research on precision positioning seeding technology, comprehensive application of sensors, Proportional Integral Derivative (PID) controllers, and other technologies, combined with modern optimization algorithms, the online dynamic calibration controls of line spacing and plant spacing are implemented. Based on the machine vision and GABP algorithm, a test platform for the seeding effect detection system is designed to provide a reference for further precision seeding operations. GA can obtain better initial network weights and thresholds and find the optimal individual through selection, crossover, and mutation operations; that is, the optimal initial weight of the Back Propagation (BP) neural network. Field experiments verify the seeding performance of the precision corn planter and the accuracy of the seeding monitoring system.Results1. The deviation between the average value of the six precision positioning seeding experiments of corn under the random disturbance signal and the ideal value of the distance is less than or equal to 0.5 cm; the deviation between the average value of the six precision positioning seeding experiments of corn under the sine wave disturbance signal (1 Hz) is less than or equal to 0.4 cm; the qualified rate of grain distance reaches 100%. 2. The precision control index, replay index, and missed index of the designed corn precision seeding intelligent control system have all reached the national standard. During the operation of the seeder, an alarm of the seeder leaking occurred, and the buzzer sounded and the screen displayed 100 times each; therefore, the reliability of the alarm system is 100%.ConclusionThe intelligent corn seeder designed based on precision positioning seeding technology can reduce the seeding rate of the seeder and ensure the stability of the seed spacing effectively. Based on the machine vision and GABP algorithm, the seeding effect detection system can provide a reference for the further realization of precision seeding operations.

Dataset Information

Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.

Publications

Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.

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