Ontology highlight
ABSTRACT:
SUBMITTER: Swatek KN
PROVIDER: S-EPMC4127189 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Swatek Kirby N KN Wilson Rashaun S RS Ahsan Nagib N Tritz Rebecca L RL Thelen Jay J JJ
The Biochemical journal 20140401 1
Plant 14-3-3 proteins are phosphorylated at multiple sites in vivo; however, the protein kinase(s) responsible are unknown. Of the 34 CPK (calcium-dependent protein kinase) paralogues in Arabidopsis thaliana, three (CPK1, CPK24 and CPK28) contain a canonical 14-3-3-binding motif. These three, in addition to CPK3, CPK6 and CPK8, were tested for activity against recombinant 14-3-3 proteins χ and ε. Using an MS-based quantitative assay we demonstrate phosphorylation of 14-3-3 χ and ε at a total of ...[more]