Ontology highlight
ABSTRACT:
SUBMITTER: Bartok E
PROVIDER: S-EPMC4127477 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Nature methods 20130106 2
Measurement of protease activity in living cells or organisms remains a challenging task. We here present a transgene-encoded biosensor that reports the proteolytic activity of caspase-1 in the course of inflammasome activation and that of other proteases in a highly sensitive and specific manner. This protease reporter is based on the biological activity of a pro-interleukin (IL)-1β-Gaussia luciferase (iGLuc) fusion construct, in which pro-IL-1β-dependent formation of protein aggregates renders ...[more]