Ontology highlight
ABSTRACT:
SUBMITTER: Repele A
PROVIDER: S-EPMC6789503 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Methods and protocols 20190725 3
Transient Luciferase reporter assays are widely used in the study of gene regulation and intracellular cell signaling. In order to control for sample-to-sample variation in luminescence arising from variability in transfection efficiency and other sources, an internal control reporter is co-transfected with the experimental reporter. The luminescence of the experimental reporter is normalized against the control by taking the ratio of the two. Here we show that this method of normalization, "rat ...[more]