?4 integrin is a regulator of leukocyte recruitment after experimental intracerebral hemorrhage.

ABSTRACT: Intracerebral hemorrhage (ICH) is swiftly followed by an inflammatory response. A key component of this response is the recruitment of leukocytes into the brain, which promotes neurological injury in rodent models. However, the mechanisms by which leukocytes transmigrate across the endothelium into the injured brain are unclear. The present study examines leukocyte adhesion molecules (?4 integrin, L-selectin, and ?L?2 integrin) on 4 leukocyte subtypes to determine which are important for leukocyte recruitment after ICH.We used the blood injection mouse model of ICH, whereby 25 ?L of blood was injected into the striatum. Flow cytometry was used to quantify leukocyte populations and adhesion molecule expression in brain and blood. An ?4 integrin-blocking antibody was administered to evaluate the contribution of ?4 integrin in leukocyte migration and neurological injury.?4 integrin was elevated on all leukocyte populations in brain after ICH, whereas L-selectin was unchanged and ?L?2 was increased only on T cells. Antagonism of ?4 resulted in decreased leukocyte transmigration and lessened neurobehavioral disability.?4 integrin is an important cell adhesion molecule involved in neuroinflammation after ICH.

SUBMITTER: Hammond MD 

PROVIDER: S-EPMC4129460 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature