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Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

ABSTRACT: Human mesenchymal stem cells (hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins (CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13659-012-0017-0 and is accessible for authorized users.

SUBMITTER: Lieder R

PROVIDER: S-EPMC4131584 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Elevated YKL-40 and low sAPP?:YKL-40 ratio in antemortem cerebrospinal fluid of patients with pathologically confirmed FTLD.

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