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In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity.

ABSTRACT: Single-nucleotide polymorphisms, either inherited or due to spontaneous DNA damage, are associated with numerous diseases. Developing tools for site-specific nucleotide modification may one day provide a way to alter disease polymorphisms. Here, we describe the in vitro selection and characterization of a new deoxyribozyme called F-8, which catalyzes nucleotide excision specifically at thymidine. Cleavage by F-8 generates 3'- and 5'-phosphate ends recognized by DNA modifying enzymes, which repair the targeted deoxyribonucleotide while maintaining the integrity of the rest of the sequence. These results illustrate the potential of DNAzymes as tools for DNA manipulation.

SUBMITTER: Wang M

PROVIDER: S-EPMC4132718 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity.

Wang Mingqi M Zhang Huafan H Zhang Wei W Zhao Yongyun Y Yasmeen Afshan A Zhou Li L Yu Xiaoqi X Tang Zhuo Z

Nucleic acids research 20140716 14

Single-nucleotide polymorphisms, either inherited or due to spontaneous DNA damage, are associated with numerous diseases. Developing tools for site-specific nucleotide modification may one day provide a way to alter disease polymorphisms. Here, we describe the in vitro selection and characterization of a new deoxyribozyme called F-8, which catalyzes nucleotide excision specifically at thymidine. Cleavage by F-8 generates 3'- and 5'-phosphate ends recognized by DNA modifying enzymes, which repai ...[more]

PMID: 25030901

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