Purification and characterization of a 1,3-?-D-glucan recognition protein from Antheraea pernyi larve that is regulated after a specific immune challenge.
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ABSTRACT: Pattern recognition receptors are known to participate in the activation of Prophenoloxidase system. In this study, a 1,3-?-D-glucan recognition protein was detected for the first time in Antheraea pernyi larvae (Ap-?GRP). Ap-?GRP was purified to 99.9% homogeneity from the hemolymph using traditional chromatographic methods. Ap-?GRP specifically bind 1,3-?-D-glucan and yeast, but not E. coli or M. luteus. The 1,3-?-D-glucan dependent phenoloxidase (PO) activity of the hemolymph inhibited by anti-Ap-?GRP antibody could be recovered by addition of purified Ap-?GRP. These results demonstrate that Ap-?GRP acts as a biosensor of 1,3-?-Dglucan to trigger the Prophenoloxidase system. A trace mount of 1,3-?-D-glucan or Ap-?GRP alone was unable to trigger the proPO system, but they both did. Ap-?GRP was specifically degraded following the activation of proPO with 1,3-?-Dglucan. These results indicate the variation in the amount of Ap-?GRP after specific immune challenge in A. pernyi hemolymph is an important regulation mechanism to immune response.
SUBMITTER: Youlei M
PROVIDER: S-EPMC4133891 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
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