Ontology highlight
ABSTRACT:
SUBMITTER: Zhang W
PROVIDER: S-EPMC4136709 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Zhang Wei W Shrestha Ruben R Buckley Rachael M RM Jewell Jamie J Bossmann Stefan H SH Stubbe JoAnne J Li Ping P
ACS chemical biology 20140616 8
Polyhydroxybutyrate (PHB) synthases catalyze the polymerization of 3-(R)-hydroxybutyrate coenzyme A (HBCoA) to produce polyoxoesters of 1-2 MDa. A substrate analogue HBCH2CoA, in which the S in HBCoA is replaced with a CH2 group, was synthesized in 13 steps using a chemoenzymatic approach in a 7.5% overall yield. Kinetic studies reveal it is a competitive inhibitor of a class I and a class III PHB synthases, with Kis of 40 and 14 μM, respectively. To probe the elongation steps of the polymerizat ...[more]