IFN-gamma and TNF associated with severe falciparum malaria infection in Saudi pregnant women.

ABSTRACT: Tumour necrosis factor (TNF) and interferon gamma (IFN-?), encoded by TNF-836 C/A (rs 1800630) and IFN-? -1616 C/T (rs2069705) genes, are key immunological mediators that are believed to both play protective and pathological roles in malaria. The aim of this study was to investigate the relationship between TNF-836 C/A and IFN-?-1616 C/T polymorphism and susceptibility to severe malaria in pregnant women.A prospective cohort (cross-sectional) study was conducted in pregnant women attending the out-patient clinic in King Fahad Specialist Hospital in Jazan (KFSHJ), with a clinical diagnosis of malaria. A total of one hundred and eighty six pregnant women were genotyped for single nucleotide polymorphism (SNP) for TNF and IFN-? using Taqman® MGB Probes. Serum cytokine concentrations were measured by sandwich ELISA method.A hospital case-control study of severe malaria in a Saudi population identified strong associations with individual single-nucleotide polymorphisms in the TNF and IFN-? genes, and defined TNF-836 C and IFN-?-1616 T genotypes and alleles which were statistically significantly associated with severe malaria infection. Furthermore, TNF-836 CC and IFN-?-1616 TT genotypes were associated with higher serum concentration of TNF and IFN-?, respectively, and with susceptibility to severe malaria.This data provides a starting point for functional and genetic analysis of the TNF and IFN-? genomic region in malaria infection affecting Saudi populations.

SUBMITTER: Nasr A 

PROVIDER: S-EPMC4137072 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature