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Tet-mediated formation of 5-hydroxymethylcytosine in RNA.

ABSTRACT: Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (?1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

SUBMITTER: Fu L

PROVIDER: S-EPMC4140497 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Tet-mediated formation of 5-hydroxymethylcytosine in RNA.

Fu Lijuan L Guerrero Candace R CR Zhong Na N Amato Nicholas J NJ Liu Yunhua Y Liu Shuo S Cai Qian Q Ji Debin D Jin Seung-Gi SG Niedernhofer Laura J LJ Pfeifer Gerd P GP Xu Guo-Liang GL Wang Yinsheng Y

Journal of the American Chemical Society 20140807 33

Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels ...[more]

PMID: 25073028

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