Ontology highlight
ABSTRACT:
SUBMITTER: Kalyoncu S
PROVIDER: S-EPMC4142072 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Kalyoncu Sibel S Hyun Jeongmin J Pai Jennifer C JC Johnson Jennifer L JL Entzminger Kevin K Jain Avni A Heaner David P DP Morales Ivan A IA Truskett Thomas M TM Maynard Jennifer A JA Lieberman Raquel L RL
Proteins 20140324 9
Protein crystallization is dependent upon, and sensitive to, the intermolecular contacts that assist in ordering proteins into a three-dimensional lattice. Here we used protein engineering and mutagenesis to affect the crystallization of single chain antibody fragments (scFvs) that recognize the EE epitope (EYMPME) with high affinity. These hypercrystallizable scFvs are under development to assist difficult proteins, such as membrane proteins, in forming crystals, by acting as crystallization ch ...[more]