Ontology highlight
ABSTRACT:
SUBMITTER: Lee J
PROVIDER: S-EPMC7181397 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Lee Jiwon J Der Bryan S BS Karamitros Christos S CS Li Wenzong W Marshall Nicholas M NM Lungu Oana I OI Miklos Aleksandr E AE Xu Jianqing J Kang Tae Hyun TH Lee Chang-Han CH Tan Bing B Hughes Randall A RA Jung Sang Taek ST Ippolito Gregory C GC Gray Jeffrey J JJ Zhang Yan Y Kuhlman Brian B Georgiou George G Ellington Andrew D AD
AIChE journal. American Institute of Chemical Engineers 20191119 3
We used the molecular modeling program Rosetta to identify clusters of amino acid substitutions in antibody fragments (scFvs and scAbs) that improve global protein stability and resistance to thermal deactivation. Using this methodology, we increased the melting temperature (T<sub>m</sub>) and resistance to heat treatment of an antibody fragment that binds to the <i>Clostridium botulinum</i> hemagglutinin protein (anti-HA33). Two designed antibody fragment variants with two amino acid replacemen ...[more]